How JE is diagnosed?

JE/AES Case Definition

 Suspected JE case:

           Acute onset of fever not more than 7 days duration.

                           and

            Change in mental status which may be

      -New onset of seizures (excluding febrile seizures) or

      -Other early clinical findings –irritability, somnolence, confusion, disorientation, coma, inability to talk or abnormal behaviour greater than that seen with usual febrile illness

Laboratory confirmed JE case:

A suspected case with any one of the following markers:

• Presence of JE virus specific lgM antibody in a single CSF sample or serum detected by IgM Capture ELISA specific for JE.
• Detection of JE virus nucleic acid detection in blood, CSF or tissue by RT PCR or any other sensitive and specific NAAT
• Detection of four-fold or greater difference in lgG antibody titre in paired sera collected 14 days apart.
• JE Virus isolation from brain tissue, CSF, serum, blood or plasma
• JE Antigen detection by immunofluorescence
• Presence of JE virus-specific lgM antibody in a single CSF/Serum sample.
• If JE-specific IgM antibodies are not present in the first serum sample, then the second sample must be obtained during 10^th -14^th day of illness.